Depending on the breed, you might be able to see the developing embryo. Shine a strong flashlight "through" the egg and look at the silhouette on the opposite side. Even a 4-6 day embryo is visible. Called "candling", some egg shells are too thick to candle. If the egg is NOT fertilized you will have a nice round silhouette (the yolk). A fertilized egg will have a red thread coming out of the yolk. Oh yeah, a dark room helps.
Otherwise, open them in another dish as Hopeak suggested.
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